Osmotic Stress Enhances Antimicrobial Activity of in Vitro Grown Microshoots of Ochradenus Baccatus Delile Against Selected microbes

Ochradenus baccatus Delile is a wild medicinal plant that produces several natural compounds with benefits. In this study, microshoots of were exposed to osmotic stress conditions consisting Murashige and Skoog solid media (MS) containing different agents (sugar types) at range concentrations (including 0.1, 0.2, 0.3 or 0.4 M). The purpose was test their effects on microshoots’ growth antimicrobial activities against selected strains bacteria one strain fungi. It has been found parameters shoot length proliferation) declined increasing sugar concentration in the media, but highest 0.4M mannitol, micorshoots could not survive. Generally, aqueous extracts stressed more effective tested microbial than methanolic most experiments. Staphylococcus aureus be affected microbe both extract types. Also, exposing had improved powers Aqueous pre-grew (0.4 M sucrose) interestingly inhibit Candida albicans minimal inhibitory (MIC) values (0.195, 0.78 mg/ml). These similar those obtained from antibiotic treatments. Other biotechnological techniques like genetic transformation are suggested also used for production elite super potential.